Current results with slow freezing and vitrification of the human oocyte

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Prospective randomized comparison of human oocyte cryopreservation with slow-rate freezing or vitrification.

OBJECTIVE To compare cryopreservation of mature human oocytes with slow-rate freezing and vitrification and determine which is most efficient at establishing a pregnancy. DESIGN Prospective randomized. SETTING Academically affiliated, private fertility center. PATIENT(S) Consenting patients with concerns about embryo cryopreservation and more than nine mature oocytes at retrieval were ran...

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Slow freezing, vitrification and ultra-rapid freezing of human embryos: a systematic review and meta-analysis.

Embryo cryopreservation is an important aspect of assisted reproduction. Many methods have been described, but they have been poorly investigated in randomized trials, highlighting the need for a systematic review of the literature. Meticulous electronic/hand searches were performed to locate randomized trials (RCT) comparing embryo cryopreservation methods. Primary outcomes were clinical pregn...

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Repeated vitrification/warming of human sperm gives better results than repeated slow programmable freezing.

In this study, we compared the effects of repeated freezing/thawing of human sperm by our in-house method of rapid freezing with slow programmable freezing. Sperm samples from 11 normozoospermic subjects were processed through density gradients and divided into three aliquots: non-frozen, rapid freezing and slow programmable freezing. Sperm in the rapid freezing group had better motility and vi...

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Slow freezing and vitrification differentially modify the gene expression profile of human metaphase II oocytes.

BACKGROUND Cryopreservation is now considered as an efficient way to store human oocytes to preserve fertility. However, little is known about the effects of this technology on oocyte gene expression. The aim of this study was to examine the effect of the two cryopreservation procedures, slow freezing and vitrification, on the gene expression profile of human metaphase II (MII) oocytes. METHO...

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Cryopreservation of human embryos by vitrification or slow freezing: a systematic review and meta-analysis.

OBJECTIVE To examine the literature systematically in order to identify prospective comparative trials answering the following question: Is vitrification of human embryos associated with a higher postthawing survival rate as compared with slow freezing? DESIGN Systematic review and meta-analysis. SETTING University-based hospital. PATIENT(S) Not applicable. INTERVENTION(S) Vitrification...

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ژورنال

عنوان ژورنال: Reproductive BioMedicine Online

سال: 2011

ISSN: 1472-6483

DOI: 10.1016/j.rbmo.2010.11.019